[Study of DNA extraction methods for testing for genetically modified organisms in soyproducts].

نویسندگان

  • Rie Moriuchi
  • Kimio Monma
  • Kunihiro Kamata
  • Akihiro Ibe
چکیده

In order to evaluate three different methods for DNA extraction (CTAB, DNeasy Plant Mini Kit and Wizard DNA Clean-up Resin system), the yields of DNA extracted from soyproducts and the copy numbers of lectin genes amplified by quantitative PCR were compared. Fermented foods, such as miso and nattou, gave poor yields of DNA and low copy numbers with any method. However atsu-age and kinugoshi-tofu gave high-quality results with all methods. Kinako gave a high yield of DNA, but poor amplification. Boiled soybeans and soymilk showed in poor amplification. It is important to choose the appropriate DNA extraction method for each product.

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عنوان ژورنال:
  • Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan

دوره 49 5  شماره 

صفحات  -

تاریخ انتشار 2008